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Captive Husbandry and Breeding of Correlophus ciliatus CAO JiaYue, CHEN ZhaoKun, ZHANG Lingna Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 91-96.   DOI: 10.19978/j.cnki.xmsy.2025.06.14 Abstract （275）      PDF （1951KB）（77）       Knowledge map    Save The crested gecko（ Correlophus ciliatus ）is an emerging specialty economic animal with a relatively short history in captive breeding but significant developmental potential. This review synthesizes critical technologies for its artificial husbandry and reproduction，aiming to provide a scientific foundation for large⁃scale farming. By analyzing habitat setup （e.g.， climbing structures， temperature/humidity control）， daily management （diet formulation，handling of shedding and tail autotomy handling），and breeding strategies（estrus detection，incubation optimization），this study systematically examines the characteristics and challenges of  C. ciliatus  aquaculture. Key findings demonstrate that habitat design must simulate arboreal niches，where container height，cork bark（e.g.， Portuguese cork wood），and humidity（70%-80%）are essential to prevent floppy tail syndrome as well as shedding complications. Reproductive success depends on breeder body weight（females ≥40 g，males ≥75 g）and incubation temperature（22.7-24.4℃），which significantly influence offspring sex ratio and viability. Disease management should prioritize calcium deficiency， gastrointestinal parasites， and environmental stressors （e.g.， head ⁃ shrinking syndrome）. In conclusions，Sustainable  C. ciliatus  farming necessitates balancing natural behavioral ecology with human intervention in captive environments. Despite rapidly growing market demand，scaling production faces challenges such as unstable hatching rates and insufficient genetic understanding of morphs traits. This review provides  practical guidelines to enhance farming efficiency and animal welfare，offering actionable insights for advancing the reptile pet industry. Reference | Related Articles | Metrics

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Study on the Effects of Compound Probiotics on Improving Laying Performance and Egg Quality of Laying Hens GOU Zhongyong, FAN Qiuli, DING Fayuan, CHENG Zhonggang, CHEN Limin, LIN Xiajing, WANG Yibing, ZHANG Sai, RUAN Dong, YE Jinling, MA Manting, JIANG Shouqun Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 1-7.   DOI: 10.19978/j.cnki.xmsy.2025.04.01 Abstract （243）      PDF （1050KB）（554）       Knowledge map    Save This study aimed to investigate the effects of dietary supplementation with different doses of compound probiotics on production performance，egg quality，reproduction��related traits and organ indices in old aged laying hens. A total of 288 Jingfen laying hens aged 68 weeks with normal egg production were selected and randomly divided into 4 groups based on similar laying rates. The basal diet was supplemented with 0，200，300，and 400 mg/kg of compound probiotics，respectively. The results showed that：dietary supplementation with compound probiotics exhibited certain trends in affecting the laying rate and daily egg mass （EM）（ P<0.10）. With the dietary supplementation level of probiotics increased，the laying rate demonstrated a significant linear increase（ P=0.047）， while daily EM showed a tendency toward linear increase（ P=0.092）. Meanwhile，the unqualified egg rate exhibited a tendency toward linear decrease（ P=0.078）. Eggs from the 300 mg/kg group had the highest eggshell strength， significantly exceeding the other 3 groups（ P<0.05）. With increasing dietary probiotic levels，Haugh unit displayed a significant quadratic effect characterized by an initial increase followed by a slight decrease（ P=0.005）. The number of dominant follicles also showed a trend of initial increase followed by decrease （ P=0.078）. Dietary probiotics significantly affected abdominal fat percentage（AF%）（ P=0.037），with the lowest value observed in the 300 mg/kg group. Based on the significance（ P values）and correlation coefficients（R2）of quadratic polynomial or broken-line models established for Haugh unit and AF% in response to probiotic dosage，and considering all experimental indicators comprehensively，the optimal dietary supplementation level of compound probiotics for aged laying hens was determined to be 300 mg/kg. Reference | Related Articles | Metrics

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Comparative study on the growth，muscle quality and benefit of Macrobrachium rosenbergii under two culture models CHEN Xiaoying, HUANG Wen, ZHOU Bingquan, SUN Yuping Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 34-39.   DOI: 10.19978/j.cnki.xmsy.2025.04.06 Abstract （223）      PDF （1096KB）（1340）       Knowledge map    Save Intensice pond culture and“cultivate Hydrilla verticillata and breeding shrimp”are the two most typical culture models of macrobrachium rosenbergii in Guangdong province. This study was invetigate to comparative the effects of two cultrue molels on growth performance，body composition，muscle amino acid composition and economic benefit of M.rosenbergii. The results showed that the final body size，condiction factor and freshness of M. rosenbergii produced under the two cultrue models including intensice pond culture and“cultivate H. verticillata and breeding shrimp”had significant difference.The contents of crude protein and calcium composition in the whole body， essential amino acids，flavorable amino acids and total amino acids in the shrimp muscle under the“cultivate H.verticillata and breeding shrimp”model were significantly higher than those under the intensice pond model. The income，net profit，input��output ratio and cost profit margin of“cultivate H. verticillata and breeding shrimp”model were obviously higher than that of the intensice pond model. These results indicated that the M. rosenbergii produced under the the“cultivate H. verticillata and breeding shrimp”model is more in line with the taste of producers and consumers，and it is suggested to adopt it in production practice and promote it. The results provided a reference for the selection of culture mode of M. rosenbergii. Reference | Related Articles | Metrics

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Verification Test of《Nutritional Requirements of Laying Ducks》 ZHANG Yuhong, ZHANG Shuyue, CHEN Wei, WANG Shuang, XIA Weiguang, JIN Chenglong, WANG Shenglin, ZHENG Chuntian, ZHANG Yanan Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 22-27.   DOI: 10.19978/j.cnki.xmsy.2025.04.04 Abstract （202）      PDF （1118KB）（103）       Knowledge map    Save In response to the urgent need for refined management and scientific feeding strategies in the current laying duck industry，a validation study was conducted on the recently published“Nutrient Requirements of Laying Ducks”（GB/T 41189－ 2021）. Diets were formulated at 95%，100%，and 105% of the standard recommended nutrient levels to investigate the applicability of current standards through comprehensive evaluation of production performance and egg quality. A total of 216 healthy 29��week�� old Longyan Shanma ducks with similar production performance were randomly allocated into three groups，with six replicates per group and twelve ducks per replicate. Three experimental diets were administered for an 18��week trial period.The results revealed that：（1）Regarding laying performance，no significant differences were observed in laying rate and feed��to��egg ratio among groups （P>0.05）. However，significant effects were detected on feed intake，average egg weight，and egg breakage rate. The lowest feed intake was recorded in the 100% group，while the highest average egg weight and lowest egg breakage rate were observed in the 105% group（P<0.05）.（2）In egg composition analysis，significant effects were found on albumen weight and eggshell weight（P< 0.05），with both parameters being higher in the 105% group，though no significant differences were detected in the proportion of egg components among groups（P>0.05）.（3）Regarding egg quality parameters，no significant effects were observed on albumen height， Haugh unit，eggshell strength，or eggshell thickness（P>0.05），while the egg shape index was significantly influenced，reaching its highest value in the 105% group （P<0.05）. In conclusion，under the experimental conditions，when evaluated against key production indicators including laying rate，feed-to-egg ratio，and egg quality，the current standard of“Nutrient Requirements of Laying Ducks”（GB/T 41189－ 2021）was demonstrated to meet production requirements. However，it is recommended that the nutrient level be adjusted to 95% when the egg breakage rate is incorporated as an additional evaluation criterion. Reference | Related Articles | Metrics

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Research Advances in the Nutrition of Energy, Protein and Amino Acids of Broilers in 2024#br# QI Zhihao, JIANG Shouqun, LAN Ruixia, ZHANG Sai Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 1-5.   DOI: 10.19978/j.cnki.xmsy.2025.06.01 Abstract （199）      PDF （1087KB）（72）       Knowledge map    Save In 2024，the research on the nutrition of energy，protein，and amino acids in broilers focused on precise nutritional regulation and sustainable development， aiming to optimize feed efficiency， production performance，and intestinal health. Studies have shown that the energy requirements of broilers are affected by multiple factors such as genetics，growth stage，environment，and feed composition. Reasonable adjustment of the energy density can significantly improve growth performance and feed conversion rate. The technology of low⁃protein diets continues to develop. Through the balance of amino acids and the supplementation of synthetic amino acids，it can maintain production performance while reducing nitrogen emissions. The precise supply of essential amino acids such as lysine and methionine is the key. The role of functional amino acids in immune regulation and intestinal health has been deeply explored，and the interaction mechanism between them and intestinal microorganisms has become a research hotspot. Reference | Related Articles | Metrics

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A Discussion on the Hatching Technology of Lion's Head Goose Eggs DU Ye, QIU Lijun Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 67-72.   DOI: 10.19978/j.cnki.xmsy.2025.04.11 Abstract （186）      PDF （1011KB）（939）       Knowledge map    Save The Lion⁃head Gooseis originally produced in Chaoshan area and is known as“the king of goose in the world”. Its size is large，growth speed is fast and economic value is high. Lion ⁃ head Goose eggs have the characteristics of large size，hard shell and thick shell membrane，and the hatching process is special. Aiming at the problems of temperature control， humidity management， ventilation exchange， egg turning frequency， egg illumination time and technique of hatching，a series of technical points and parameters were put forward in order to improve hatching rate and healthy rate of Lion⁃head Goose eggs. Provide scientific guidance for breeders，thereby enhancing economic and social benefits. Reference | Related Articles | Metrics

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Analyzing the Future of the Cattle Breeding Industry in Guangdong Province from the Perspective of the National Beef Cattle Industry Development TONG Xiong, MIN Li, ZHANG Zhifei, TAN Sheng, MA Xinyan, LI Dagang Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 105-110.   DOI: 10.19978/j.cnki.xmsy.2025.04.16 Abstract （172）      PDF （2520KB）（695）       Knowledge map    Save This study delineates the evolving dynamics between cattle inventory and beef production in China， while highlighting the upward trajectory of domestic beef consumption and the increasing reliance on imports. From the perspective of germplasm resource introduction，it examines the influence of live cattle and frozen semen imports on the broader development of the beef industry. Integrating insights from the market circulation system，the paper further dissects the volatility patterns within the fresh beef consumption market. Focusing specifically on Guangdong Province，the paper evaluates the current state of the beef cattle sector through three analytical lenses：the imbalance in supply and demand，the degree of large⁃scale farming，and the distinctive characteristics of regional dairy cattle farming models. It identifies critical challenges facing the provincial cattle breeding industry，including a shortage of high⁃quality genetic stock，weak foundational infrastructure in breeding systems，misalignment between breeding objectives and the diversified demands of the market，and insufficient protection and utilization of indigenous cattle germplasm resources. Building upon the analysis of these structural bottlenecks，the paper proposes a systematic development pathway. This includes the establishment of a province⁃wide elite breeding system，targeted enhancement of genetic improvement technologies，the development of intelligent innovation platforms for germplasm advancement， and the identification of genetic markers associated with desirable traits. Together，these measures aim to provide a scientific foundation for the high⁃quality and sustainable development of Guangdong’s beef cattle breeding industry. Reference | Related Articles | Metrics

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Surveillance and Analysis of Avian Leukosis in Milin Tibetan Chicken XU Xinchun, HE Jingyi, YAN Xia, CHEN Peng, JIA Shaoyan, JI Jian, LUO Chenglong Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 40-44.   DOI: 10.19978/j.cnki.xmsy.2025.04.07 Abstract （168）      PDF （1516KB）（1250）       Knowledge map    Save To explore the detection effect of different detection methods and the feasibility of the combination of avian leukemia virus（ALV）infection and AL purification，the study in chicken，using ALV p27 antigen ELISA method and ALV⁃JPCR nucleic acid detection method，from 2020 to 2024 a total of 1 333 randomly collected whole blood samples for ALV detection analysis. The results showed that in Tibetan chickens，the positive rate of ALV p27 antigen ELISA test was 15.42%，ALV⁃JPCR nucleic acid test was 2.49%. The total positive rate of the two detection methods was 17.23%，among which only 3 individuals were positive for the two detection methods，accounting for 3.95% of the total positive individuals. In addition，since AL purification in 2020 to 2024，the AL positive rate of milin Tibetan chickens decreased from 17.23% to 0 between 2020 and 2024. The results show that there are mixed infection of ALV⁃J subgroup and other subgroups in the flock；the combination of the two methods can greatly improve the ALV detection rate and can provide reference for the actual purification work；the AL purification of milin Tibetan chicken group is significant，which basically realizes the effective control of AL in the flock，which can provide data support for the AL prevention and control purification of this species. Reference | Related Articles | Metrics

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Establishment and Application of a Double Fluorescence Quantitative Polymerase Chain Reaction Detection Method for Novel Goose Astrovirus Types 1 and 2 XU Hang, LIU Mengfan, YIN Zhenghao, LU Yongkun, LI Yuze, LUO Haojian, LIN Junjie, WANG Yiqiao, LIU Yingying, MEI Kun, HUANG Shujian Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 83-93.   DOI: 10.19978/j.cnki.xmsy.2025.04.14 Abstract （165）      PDF （4086KB）（352）       Knowledge map    Save In order to develop a credible dual fluorescence quantitative polymerase chain reaction（qPCR）for detecting novel goose astrovirus type 1（NGAstV⁃1）and type 2（NGAstV⁃2），primers were designed to establish a dual fluorescence qPCR detection method using ORF2 protein of NGAstV⁃1 and NGAstV⁃2，and 36 samples of goose liver and kidney tissue suspected to be infected with NGAstV⁃1 and NGAstV⁃2 were evaluated in this study. The test results showed that the correlation coefficients of qPCR were 0.997 and were negative for novel duck reovirus （NDRV），avian influenza virus（AIV），fowl adenovirus（FAdV），and goose parvovirus（GPV）. The minimum detection limit was 1.12 × 101 copies/μL. The reproducibility test results indicate good reproducibility，with a coefficient of variation between groups of less than 0.8% and a coefficient of variation within groups of less than 0.5%. It can be seen that the qPCR can efficiently and specifically detect NGAstV⁃1 and NGAstV⁃2，providing a feasible method for NGAstV monitoring. Reference | Related Articles | Metrics

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Comparative Analysis of Morphology Characteristics，Growth and Reproductive Performance among Yueyang Pigeon，Shiqi Pigeon#br# and the Mimas Strain of European Meat Pigeon#br# XU Dong, GUO Shiqi, XU Kunjie, CHEN Yitian, WU ZhongPing, FANG Quanmin, YU Lian, LIANG Yayan, ZHANG Xumeng Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 69-78.   DOI: 10.19978/j.cnki.xmsy.2025.06.11 Abstract （163）      PDF （2120KB）（22）       Knowledge map    Save As China's meat pigeon industry has become the fourth largest poultry sector following chickens， ducks，and geese，the Yueyang pigeon，a unique genetic resource of Yueyang County in Hunan Province，boasts an annual slaughter output of over 10 million birds. However，there is a lack of systematic research on its breed characteristics. To evaluate the differences between Yueyang pigeon and other breeds，this study conducted a comparative analysis of the morphology traits，growth performance，and reproductive performance of Yueyang pigeon， Shiqi pigeon，and the European meat pigeon Mimas strain. The results revealed significant differences in body shape， head shape，legs，and feather color among the three breeds. Regarding growth performance，the Mimas strain exhibited extremely significant advantages in body weight，shank circumference，and chest depth over the other two breeds（P<0.01）. The body slant length of Yueyang pigeon was extremely significantly greater than that of the other two（P<0.01）. In male pigeons，the Mimas strain showed extremely significantly greater shank length and chest width than the other breeds（P<0.01），and its neck circumference was extremely significantly larger than that of Yueyang pigeon（P<0.01）. In terms of slaughter performance，the Mimas strain had the highest live body weight at slaughter， and its semi⁃evisceration yield was extremely significantly higher than that of Yueyang pigeon（ P<0.01）. The full⁃ evisceration yield of Yueyang pigeon was significantly higher than that of the other two breeds（ P<0.05），and its abdominal fat percentage was extremely significantly higher than that of Shiqi pigeon（ P<0.01）. For reproductive performance，the number of clutches and the number of chicks hatched of Shiqi pigeon were extremely significantly lower than those of the Mimas strain（ P<0.01），but the annual squab production of both Shiqi and Mimas was extremely significantly higher than that of Yueyang pigeon（ P<0.01）. In meat and egg quality，Shiqi and Mimas pigeons were superior in egg weight and pH value，while Yueyang pigeon demonstrated better performance in meat redness（a* value），shear force，and yolk color. In conclusion，significant or extremely significant differences were observed in growth performance，reproductive performance，and meat and egg quality among the three pigeon breeds. This study clarifies the breed characteristics and production potential of Yueyang pigeon，providing data and theoretical references for the conservation of its germplasm resources，optimization of breeding programs，and high⁃ quality development of the industry. Reference | Related Articles | Metrics

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Isolation，Identification，and Genetic Evolutionary Analysis of a Goose Parvovirus Strain PENG shan, WANG chao, ZHU Wanjun, ZHANG Jipei, CHEN Jidang Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 94-104.   DOI: 10.19978/j.cnki.xmsy.2025.04.15 Abstract （161）      PDF （8044KB）（62）       Knowledge map    Save Goose parvovirus（GPV）is the causative agent of Gosling plague，a highly lethal infectious disease in goslings under 20 days of age，while juvenile geese often exhibit subclinical infections that are frequently overlooked. In November 2024，an acute outbreak occurred in a flock of juvenile geese（56 days old）at a goose farm in Jieyang， Guangdong Province. To identify the cause of the disease，standard necropsy procedures were conducted on the submitted dead geese，and hepatic，splenic，and intestinal tissue samples were aseptically collected. Pathogen nucleic acids of common viral infectious diseases in geese were detected using PCR/RT⁃PCR assays. The GPV⁃positive tissue samples homogenized and inoculated into goose embryos. Subsequently，PCR⁃positive allantois fluid samples were subjected to full⁃genome cloning and sequencing，followed by phylogenetic and molecular evolutionary analysis based on the obtained sequence data. The results indicated that the submitted tissue samples tested positive for goose parvovirus（GPV）nucleic acid by PCR，while showing negative results for goose circovirus（GoCV），goose astrovirus （GoAstV），Newcastle disease virus（NDV），and Tembusu virus（TMUV）. After inoculation of the GPV⁃positive tissue homogenate into goose embryos，embryo death with severe hemorrhage was observed，and subsequent PCR testing confirmed GPV positivity in the allantois fluid. Genome sequencing revealed that the viral strain possesses a complete genome of 5 028 bp，consisting of inverted terminal repeat（ITR）regions along with non⁃structural（NS）and capsid（VP）genes. The strain was designated as GPV/GD/3121/2024. Similarity analysis showed that this isolated strain had the highest similarity to the DY16 strain in both the NS1 gene（99.4%）and VP1（95.9%）. The GPV/GD/ 3121/2024 strain exhibits a high whole⁃genome sequence similarity of 97.5% with recent GPV strains isolated in China，including the DY16，RC16，and HB⁃2019 strains. Phylogenetic analysis based on the complete genome，non⁃ structural protein NS1，and structural protein VP1 sequences consistently demonstrated that the isolate clustered within a major clade sharing close genetic relationships with European vaccine strain VG32/1）domestic vaccine strains（GDaGPV，SYG61v），and Taiwanese vaccine strain 82⁃0321V. Additionally，compared to classical GPV strains，GPV/GD/3121/2024 harbored three unique nucleotide mutations in the VP1 gene（A2496T，G2515C，and A2772G），resulting in amino acid substitutions V38L，S178T，and Y491H in the VP1 structural protein. This study confirmed that the lethal outbreak in juvenile geese was caused by GPV infection. The causative strain was successfully isolated and characterized through comprehensive genetic and evolutionary analyses. Furthermore，we summarized the current epidemiological status of GPV in China. These findings provide valuable reference data for understanding the biological characteristics of GPV and formulating scientific control strategies. Reference | Related Articles | Metrics

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Evaluation of Heat Tolerance，Detection and Analysis of Physiological and Biochemical Indexes and Immune Function of Hu Sheep WANG Fan, ZOU Jianwei, MO Zhihua, HAN Wei, YANG Duohu, HU Deqiang, NONG Huating, WEI Yingming, ZHANG Ruimen, HUANG Yanna, JIANG Qinyang Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 8-16.   DOI: 10.19978/j.cnki.xmsy.2025.04.02 Abstract （159）      PDF （1161KB）（469）       Knowledge map    Save The purpose of this experiment was to explore the changes in physiological indices， serum biochemical indices and immune function of Hu sheep with different heat tolerance，and to provide reference for  analyzing the heat tolerance mechanism of Hu sheep and formulating strategies to alleviate heat stress of sheep. From July 1 to August 20，under the condition of an average THI（Temperature⁃Humidity Index）of 85.5，a total of 811 multiparous，non⁃pregnant ewes with consistent parity（all having given birth twice），good health status，and identical feeding management were selected and raised collectively in a single shed. Indicators such as respiratory rate，rectal temperature，and heart rate were measured. Using principal component analysis and membership function analysis，heat⁃resistant（HR）Hu sheep（n=30）and heat⁃sensitive（HS）Hu sheep （n=30）were identified. The results showed that the respiratory frequency，rectal temperature and heart rate of Hu sheep in HS group were significantly higher than those in HR group（P<0.01）. WBC and HGB in the blood routine of Hu sheep in HR group were significantly higher than those in HS group（P<0.01），and LYM was significantly higher than that in HS group（P<0.05）. The contents of serum HSP70 and HSP90 in HR group were significantly higher than those in HS group（P<0.01）. The contents of T3， T4 and COR in HR group were significantly higher than those in HS group（P<0.01）. The concentrations of TNF⁃α，IL⁃1β and IL⁃6 in HR group were also significantly higher than those in HS group（P<0.01）. In conclusion，compared with HS Hu sheep，HR Hu sheep exhibit stronger heat stress resistance and immune capacity. Reference | Related Articles | Metrics

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Fitting of Early Growth Curves and Correlation Analysis between Body Weight and Body Size Traits in Fast-growing Type Magang Geese#br# LUO Lei, PENG Siyue, ZHANG Kaite, CHEN Wenjun, LIN Rihui, GUO Pengyun, HUANG Zhirong, ZHANG Liyun, LUO Maojun, LI Xiujin, ZHANG Xumeng, HUANG Yunmao, TIAN Yunbo, WU Zhongping Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 28-33.   DOI: 10.19978/j.cnki.xmsy.2025.04.05 Abstract （158）      PDF （1325KB）（93）       Knowledge map    Save To investigate the early growth patterns of fast ��growing Magang geese，this study employed three nonlinear growth curve models（Logistic，Gompertz，and Von Bertalanffy）to analyze the body weights of 264 fast- growing Magang geese（130 males and 134 females）from 1 to 90 days of age. Additionally，a correlation analysis was conducted between body weight and various body measurements at 70 days of age. The results revealed that the Logistic model was the optimal growth curve model for male geese，with an R2 value of 0.998. The inflection point for male geese occurred at 39.9 days，with a corresponding body weight of 2 272.05 g，and the estimated mature weight was 4 434.85 g. For female geese，the best-fitting model was the Gompertz model（ R2=0.999），with an inflection point at 31.5 days，an inflection point weight of 1 564.31 g，and a mature weight of 3 888.30 g. A significant positive correlation（ P<0.05）was observed between body weight and body measurements at 70 days of age，with shank circumference demonstrating the strongest correlation. Therefore，it is recommended that shank circumference be used as a reference for early weight selection in fast-growing Magang geese. To optimize the early growth potential of both male and female geese，it is suggested that male geese should receive enhanced nutritional levels during the first 40 days of age，while female geese should be provided with adequate nutrition during the first 32 days of age. Reference | Related Articles | Metrics

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Network Pharmacology Analysis and Experimental Investigation of the Anti ⁃ Inflammatory Effects of Houttuynia Cordata，#br# Lithospermum Erythrorhizon，and Isatis Indigotica#br# Yue Zipeng, HE Yinghui, HAO Linyao, Huo Shuying, LI Yurong, LI Shupeng Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 7-16.   DOI: 10.19978/j.cnki.xmsy.2025.05.02 Abstract （156）      PDF （6904KB）（36）       Knowledge map    Save To investigate the anti ⁃ inflammatory effects of the combination of Houttuynia cordata，Rubia cordifolia，and Isatis indigotica on macrophage RAW264.7 cells through network pharmacology，molecular docking， and experimental verification methods. The active components and related target sites of Houttuynia cordata，Rubia cordifolia，and Isatis indigotica were identified using TCMSP，ETCM，UniProt，and SwissTargetPrediction databases；component⁃inflammation target network diagram was drawn using Cytoscape 3.6.1 software，and GO function and KEGG pathway enrichment analyses were performed；molecular docking was conducted using AutoDock software. The mRNA secretion of TNF⁃α，IL1⁃β，and IL⁃6 in macrophage RAW264.7 cells was detected by real⁃time fluorescent quantitative PCR to evaluate the anti ⁃ inflammatory effect of the herbal mixture. The anti ⁃ inflammatory effect of Houttuynia cordata，Rubia cordifolia，and Isatis indigotica combination was detected by mouse ear swelling test. A total of 702 active components were obtained for Houttuynia cordata，Rubia cordifolia，and Isatis indigotica， including scutellarin，corindone，and kaempferol，etc.；675 drug⁃inflammation intersection targets were obtained， including IL6，IL1β，and serine/threonine protein kinase（AKT1），etc.；GO function enrichment analysis showed 186，36，and 86 entries；KEGG result analysis yielded 189 pathways. Molecular docking results showed that the active ingredients of the drugs had good binding ability with the main targets. In vitro experiments demonstrated that the three herbal medicines could effectively inhibit the caused by Lipopolysaccharide（LPS），with pathological increases of inflammatory factors such as IL⁃6，TNF⁃α，and IL⁃1β. The combination of Houttuynia cordata，Rubia cordifolia，and Isatis indigotica inhibited the ear swelling of mice caused by dexamethasone at 47.61%，which was significantly different from the model group，indicating that the herbal mixture can exert anti⁃inflammatory effects. The good anti⁃inflammatory effect of the combination of Houttuynia cordata，Rubia cordifolia，and Isatis indigotica is demonstrated by network pharmacology analysis and experimental results，suggesting that it exerts anti⁃inflammatory effects through multiple components，multiple pathways，and multiple targets. Reference | Related Articles | Metrics

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Research Progress on Available Energy and Amino Acid Digestibility of Wheat Bran in Pigs ZHANG Jianjian, WANG Li, YANG Xuefen, LI Ping Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 51-60.   DOI: 10.19978/j.cnki.xmsy.2025.04.09 Abstract （155）      PDF （1232KB）（238）       Knowledge map    Save Wheat is the second largest food crop in the world，ranking third in China. The crude protein and crude fiber content of wheat bran produced by its by⁃product processing is higher，and the available energy value is slightly lower than that of corn，which is one of the important energy feed raw materials. However，due to the different sources，processing methods and anti⁃nutrition factors，the nutritional value of wheat bran is very different，which restricts its feeding value. Therefore，this paper mainly reviewed the differences of nutritional components of different wheat bran，the available energy and amino acid digestibility of pigs and their applications in pig production. Reference | Related Articles | Metrics

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Diagnosis and Treatment of Urinary Obstruction in a Neutered Male Cat DONG Chunlin, YE Junjie, SONG Jia, LIU Shuyang Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 100-105.   DOI: 10.19978/j.cnki.xmsy.2025.06.16 Abstract （150）      PDF （2570KB）（1293）       Knowledge map    Save This study aims to explore the diagnostic and therapeutic methods for feline urinary retention. As a common manifestation of Feline Lower Urinary Tract Disease（FLUTD），feline urinary retention can lead to systemic infection or even death in severe cases. In this case，feline urinary retention was initially suspected through inspection，inquiry，and palpation；further confirmed by imaging examinations including DR and abdominal color Doppler ultrasound；and finally diagnosed based on the results of blood routine，biochemical indicators，and coagulation function tests. After 3 days of conservative treatment，the cat's symptoms showed no significant improvement，so perineal urethrostomy was performed. The cat achieved good postoperative recovery and a favorable prognosis. This case indicates that for urinary retention cases unresponsive to conservative treatment，perineal urethrostomy can effectively improve the quality of life of affected cats，providing valuable reference for clinical practice. Reference | Related Articles | Metrics

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Research on the Inhibitory Effect of Rose Essential Oil and Active Ingredients on Three Aquatic - derived Bacteriaincluding Streptococcus Iniae XU Yibing, LIN Zhiyu, FENG Kaien, LIANG Kaiqi, TUO Jiangyu, LI Jialing, LIU Siping, LIU xiaoxi Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 45-50.   DOI: 10.19978/j.cnki.xmsy.2025.04.08 Abstract （149）      PDF （1351KB）（98）       Knowledge map    Save To experimentally investigate the in vitro inhibitory zonesize and minimum inhibitory concentration of rose essential oil and its antimicrobial active ingredients citronellol，geraniol and nerol against three aquatic pathogens，namely Vibrio parahaemolyticus（ V. parahaemolyticu），Streptococcus iniae（ S.iniae），and Vibrio harveyi（ V. harveyi）. The inhibitory effects of rose essential oil and its antibacterial components（citronellol，geraniol and nerol） on three aquatic pathogenic bacteria（ V. parahaemolyticu， S. iniae and V. harveyi）was tested. The diameter of the inhibition zones of rose essential oil and its antibacterial agents was determined using the methods of punch-hole and cross-validation. The minimum inhibitory concentrations（MIC）of rose essential oil and its antibacterial compounds were determined using the 96-well plate microdilution method.Finally，the fractional inhibitory concentration（FIC） indices of the three active ingredients were determined using the micro-broth chessboard cross-validation method. The results demonstrated that rose essential oil and its active ingredients exhibited antibacterial activity against V. parahaemolyticu， S. iniae and V. harveyi. Among these，citronellol demonstrated the most pronounced antibacterial effect. The three rose essential oil active ingredients were tested in combination with one another to evaluate their joint antibacterial effect，with the findings indicating that the combined effect was either irrelevant or antagonistic.Rose essential oil and its active ingredients have been demonstrated to exert notable bacteriostatic effects on aquatic pathogenic bacteria. However，the simultaneous application of its active ingredients in a clinical setting is not recommended.This study will provide a theoretical foundation for the future application of the antibacterial effects of rose essential oil in aquaculture Reference | Related Articles | Metrics

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Effects of Compound Nutritional Health Sand on the Slaughter Performance，Biochemical Indicators，and Intestinal Health of Squab Pigeon#br# LU Haopeng, LI Ziying, ZENG Fanming, ZHENG Shiqi, ZHANG Wei, ZHANG Yanlin, HE Yanle, SONG Mengping, GOU Zhongyong, WANG Jun, LIANG Yayan, HUANG Yanhua, PENG Jie, YAN Kai Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 24-32.   DOI: 10.19978/j.cnki.xmsy.2025.05.04 Abstract （147）      PDF （1409KB）（541）       Knowledge map    Save This study aimed to evaluate the effects of compound nutritional health sand on slaughter performance，meat quality，carbohydrate and lipid metabolism，oxidative stress，intestinal morphology，and intestinal enzyme activity in pigeons. For this experiment，96 pairs of Mimas pigeons with similar age，weight，and reproductive performance were selected pigeons. They were randomly divided into two treatment groups. Each group contained 8 replicates，each replicate consisting of 6 pairs of pigeons，and each pair of pigeons rearing 4 squabs. The control group was fed with basic health sand，while the experimental group was fed with compound nutritional health sand. The experimental period was 45 days（17 days of hatching，21 days of lactation，and 7 days of rest）. The results showed the following：Compared with the control group，the crypt depth of the jejunum in the squabs of the experimental group exhibited significantly reduced，and the villus⁃to⁃crypt ratio was significantly increased（ P<0.05），while there were no significant changes in the villus height，muscle layer thickness，and villus width of the jejunum（ P>0.05）. Compared with the control group，the content of plasma albumin（Alb，Albumin）in the squabs of the experimental group was significantly increased（ P<0.05），and there were no significant changes in the content of plasma glucose （Glucose，GLU），urea nitrogen（Urea nitrogen，BUN），total protein（Total Protein，TP），uric acid（Uric acid， UA），total cholesterol（Total Cholesterol，T⁃CHO），and triglycerides（Total Triglyceride，TG）（ P>0.05）. Compared with the control group，the activity of amylase（Amylase，AMY）and total superoxide dismutase（Total Super Oxide Dismutase，T⁃SOD）in the jejunum of the squabs of the experimental group was significantly increased（ P<0.01）， while there were no significant changes in the activity of total antioxidant capacity（Total anti⁃oxidant status，T⁃AOC）， chymotrypsin（Chymotrypsin，Chy），maltase（Maltase，MDH），catalase（Catalase，CAT），and the content of malondialdehyde （Malonaldehyde， MDA） and hydrogen peroxide （Hydrogen peroxide， H2O2）（ P<0.05）. In conclusion，compound nutritional health sand promotes the development of the intestinal tract in pigeons，enhances intestinal health，and holds potential application value in production. Reference | Related Articles | Metrics

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Comprehensive Prevention and Control Strategies for Porcine Epidemic Diarrhea in Large⁃Scale Swine Farms GENG Rui, XU Zhichao, KANG Qixiang, HU Guangli, SHEN Hanqin, CAO Yongchang Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 83-90.   DOI: 10.19978/j.cnki.xmsy.2025.05.13 Abstract （146）      PDF （1075KB）（529）       Knowledge map    Save Porcine epidemic diarrhea（PED），caused by the porcine epidemic diarrhea virus（PEDV），is a highly contagious and lethal infectious disease in pigs. The virus primarily targets the intestinal epithelium，leading to acute diarrhea and dehydration. Since the emergence of highly pathogenic PEDV variants in China in late 2010，PEDV has spread globally，causing significant economic losses on the global swine industry. This review systematically summarizes the current epidemiological trends，genetic variations and hazards of PEDV. Focusing on the three fundamental components of infection source，transmission route，and susceptible animal of infectious diseases，this paper highlights the challenges in current PED prevention and control，and proposes a comprehensive PED prevention and control strategy emphasizing enhanced biosecurity measures and scientifically immunization programs. Reference | Related Articles | Metrics

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2024 Annual Investigation of Insect Vectors in Equine Endemic Areas in Conghua District of Guangzhou City ZHONG Jiangguo, HUANG Junjie, CHI Shihong, LI Yuze, LIN Junjie, WANG Yiqiao, LUO Haojian, LIU Yingying, MEI Kun, HUANG Shujian Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 102-108.   DOI: 10.19978/j.cnki.xmsy.2025.05.16 Abstract （146）      PDF （1674KB）（60）       Knowledge map    Save To understand the presence，distribution，activity patterns，and potential to carry pathogens related to diseases such as equine piroplasmosis，Japanese encephalitis，vesicular stomatitis，Trypanosomiasis，West Nile virus，African horse sickness，equine infectious anemia，and equine encephalomyelitis（both Eastern and Western forms），in the unregulated equine disease zones of Conghua District in Guangzhou，we established a monitoring framework. Taking Conghua District as the core area，set up 8 surrounding districts and counties as monitoring areas to capture mosquitoes and insects for monitoring. Through 3 surveys in May，July and September，8，177 mosquitoes， 484 tabanids，655 midges and 529 ticks were caught. Among them，mosquitoes belong to 4 subfamilies，8 genera and 28 species，tabanids belong to 2 subfamilies，2 genera and 2 species，midges belong to 2 subfamilies，2 genera and 2 species，ticks belong to 1 subfamily，1 genus and 1 species. The dominant mosquitoes were culex brevicornis，Culex pseudosquemiculatus， Culex pipiens quinquefasciatus， chironomus， Tabanidae， Ceratopogonidae and Ixodes fanghead（Ixodes）. In May，3，678 mosquitoes were collected，with a mosquito density of 2.36 mosquitoes per light trap hour. In July，2，562 mosquitoes were captured，yielding a density of 1.64 mosquitoes per light trap hour. In September，1，937 mosquitoes were collected，with a density of 1.24 mosquitoes per light trap hour. The number and density of mosquitoes decreased from March to September. The average density of Tabanids was 6.2/field，average density of midges was 8.3/field，and average density of ticks was 8.3/field. Reference | Related Articles | Metrics

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Effects of Cherry Valley Male Ducks on Nutrient Digestion and Amino Acid Utilization of Hydrolyzed Feather Meal ZHU Junrong, ZHANG Haipeng, XIAO Kaige, LYU Yantao, ZHENG Chuntian, WANG Shuang, ZHANG Yanan, WANG Shenglin, JIN Chenglong, HUANG Xuebing, LI Kaichao, CHEN Wei, XIA Weiguang Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 1-6.   DOI: 10.19978/j.cnki.xmsy.2025.05.01 Abstract （145）      PDF （1198KB）（78）       Knowledge map    Save This study aimed to evaluate the nutrient digestibility and amino acid utilization of hydrolyzed feather meal produced in Fujian，Shandong，and Guangdong provinces in male Cherry Valley ducks at sexual maturity. Forty�� eight healthy adult male Cherry Valley ducks were randomly divided into four groups with 12 ducks in each group. They were fed with 100% corn starch（control group）and 85% corn starch + 15% hydrolyzed feather meal（Fujian， Shandong，and Guangdong），respectively. Through the metabolic trial of forced feeding��total feces collection，the excreta were collected，and the quality，moisture，dry matter，crude protein，amino acids，calcium and phosphorus of feed and excreta were determined. There were differences in the contents of dry matter，crude protein and true amino acids in hydrolyzed feather meal originating from different regions. The hydrolyzed feather meal produced in Fujian showed the highest contents of dry matter（93.7%） and crude protein（83.4%）. The crude protein contents of hydrolyzed feather meal produced in Shandong and Guangdong were slightly higher than those of Fujian，but the differences in crude protein were not significant（P>0.05）. The true amino acid availability of aspartic acid，threonine and glutamic acid in Fujian hydrolyzed feather meal was higher than that in Shandong and Guangdong（P<0.01）. After force- feeding the diets containing hydrolyzed feather meal produced in Fujian，Shandong and Guangdong，no differences was observed in the weights of feces and fecal contents of dry matter，crude protein，calcium，and phosphorus. The digestibility of dry matter，protein and nitrogen in the feces of Cherry Valley ducks fed hydrolyzed feather meal from different production areas did not show significant differences among Fujian，Shandong and Guangdong（P>0.05）. There were few differences in other nutrients except amino acids in hydrolyzed feather meal from different production areas. The amino acid contents of hydrolyzed feather meal in Fujian were higher，especially the true availabilities of aspartic acid and threonine was significantly better than those of hydrolyzed feather powder in Shandong and Guangdong. The digestibility of dry matter and crude protein of hydrolyzed feather meal among Fujian， Shandong，and Guangdong was less significant. Reference | Related Articles | Metrics

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The Application of Somatic Cell Nuclear Transfer Technology in Conserving Genetic Resources of Lantang Pigs ZHOU Rong, SU Qiaoyun, LUO Lvhua, MAI Ranbiao, JI Hongmei, Yu Wanxian, LIANG Huanlian, SHI Junsong, ZOU Xian, MENG Fangming, CAI Gengyuan Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 17-21.   DOI: 10.19978/j.cnki.xmsy.2025.04.03 Abstract （144）      PDF （1580KB）（401）       Knowledge map    Save This study aimed to clone the Lantang pig using somatic cell nuclear transfer technology，offering a novel and efficient approach for preserving the genetic resources of exceptional local and endangered pig breeds in China. We compared the tissue block method and collagenase digestion method to establish Lantang pig somatic cell lines. Additionally，we analyzed the developmental outcomes of cloned embryos created from Lantang pig donor cells of different genders in vitro and transplanted them in vivo. The results indicated that the collagenase method yielded cells more rapidly and in higher quantities compared to the tissue block method. While there were no notable differences in cleavage rates and total blastocyst cell counts between cloned embryos of different genders in vitro，the blastocyst rate of male embryos was significantly higher than that of female embryos（P<0.05）. Through in vivo transfer of cloned embryos from Lantang pigs in three separate batches，we successfully obtained four healthy cloned piglets. This study marks the first successful case of somatic cell cloning of the Lantang pig in China，providing a dependable technical tool for preserving the genetic resources of this breed. Reference | Related Articles | Metrics

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Artificial Incubation and Hand Rearing of the Common Moorhen（ Gallinula chloropus） LI Yiyuan, HE Yongxin, OU Zitao, YE Caiqun, HU Yuchan Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 73-77.   DOI: 10.19978/j.cnki.xmsy.2025.04.12 Abstract （144）      PDF （1293KB）（443）       Knowledge map    Save By simulating the natural incubation environment of the Common Moorhen（Gallinula chloropus）， including the regulation of temperature and humidity，and providing appropriate rearing and care of the hatchlings， this study aimed to explore artificial incubation and chick-rearing methods for the species. The results demonstrated that the artificial incubation period of the Common Moorhen ranged from 18 to 20 days，with the majority hatching at 19 days. The average weight and dimensions of Common Moorhen eggs were（18.33±1.00）g，（42.14±0.79）mm × （29.24±0.64）mm respectively，with an egg shape index of（1.45±0.03）. Under+incubation conditions of 37.8℃ temperature and 55% relative humidity，the hatching rate reached 88.89% . Fertilized Common Moorhen eggs exhibited varying degrees of weight loss during incubation，which exhibited a linear relationship with incubation days， as described by the linear regression equation y=- 0.1133x + 18.339（R2=0.998）. The growth and development of Common Moorhen chicks conforms to the Logistic，Gomertz，and Von Bertalanffy growth curve models，with the curve fitting degrees R2 being 0.981，0.963，and 0.955，respectively. Among these，the Logistic model exhibited the highest fitting degree. The Logistic equation is y=172.68（/ 1+21.587e-0.132609t）with a curve fit of R2=0.981. The inflection point mass A/2 was 86.34 g，and the inflection point age lnB/k was 23.16 days. These findings provide important theoretical foundation and practical insights for guiding the artificial incubation and chick rearing management of Common Moorhens，thus offering practical technical support for improving their breeding efficiency. Reference | Related Articles | Metrics

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Development of a Big��data��driven Early Warning System for Animal Diseases Wang Neng, Han Xianghong, Yan Beibei Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 114-120.   DOI: 10.19978/j.cnki.xmsy.2025.05.18 Abstract （141）      PDF （3220KB）（26）       Knowledge map    Save With the rapid growth of the township animal husbandry industry，the frequent outbreak of animal diseases seriously threatens the safety of the industry and the income of farmers. Establishing an early warning system for animal diseases based on big data analysis is therefore essential. Focusing on the actual needs of township animal husbandry and veterinary stations，this paper proposes a method for building an early warning system for animal diseases based on big data analysis. By integrating multi ⁃ source information such as daily farm data，epidemic detection data，and environmental monitoring data，and using data analytics methods suitable for grassroots level，a straightforward and practical early warning index system and prediction model are established. This system enables early detection and warning of animal diseases，thereby enhancing prevention and control capabilities at the township level，reducing breeding losses，and promoting the healthy development of the animal husbandry as well as public health safety. Reference | Related Articles | Metrics

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Application of Pogostemon cablin and Its Compound Preparation in Livestock Production DENG Dongyan, CAO Shuting, XIONG Yunxia, YANG Xuefen, GAO Kaiguo, WANG Li Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 61-66.   DOI: 10.19978/j.cnki.xmsy.2025.04.10 Abstract （139）      PDF （1028KB）（117）       Knowledge map    Save Climate change directly or indirectly affects the health of livestock and poultry，leading to challenges such as heat stress，metabolic disorders，oxidative stress，and immunosuppression，which pose significant threats to the development of the livestock industry. Pogostemon cablin，commonly known as Patchouli，is a traditional Chinese herb known for its anti⁃inflammatory，antioxidant，and antidiarrheal properties. When used as a feed additive，either alone or in combination with other herbal formulations，it has been shown to alleviate heat stress in livestock and poultry， promote gastrointestinal health， and enhance antioxidant capacity to varying degrees. This article systematically reviews the physicochemical properties of Pogostemon cablin and its compound preparation，as well as their applications in livestock production. The findings aim to provide a reference for further promoting the use of Pogostemon cablin in animal production systems. Reference | Related Articles | Metrics

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Analysis of Pathogenic Factors and Comprehensive Prevention and Control Measures of Salpingitis in Laying Hens WANG Shenglin, HUO Xinghua, SUN Jing, LI Yanfang, CAO Xiuqin, CHEN Haijun, CHEN Shan, Qian Huiwen Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 29-34.   DOI: 10.19978/j.cnki.xmsy.2025.06.05 Abstract （134）      PDF （1094KB）（419）       Knowledge map    Save Salpingitis is a common multiple disease during the laying period，which can lead to a decline in the laying rate，with symptoms including the absence of an laying peak period，deterioration in eggshell quality，and a decrease in egg quality. The etiological factors contributing to salpingitis are complex，mainly associated with pathogenic bacterial infections， inappropriate feeding management， nutritional deficiencies， substandard environmental hygiene，and disease prevention and control strategies. Starting from the hazards of salpingitis，this article outlines the potential pathogenic factors，and proposes comprehensive measures that need to be taken in the production management process of laying hens，in order to provide a reference for peers in the laying hen industry to effectively prevent and control salpingitis. Reference | Related Articles | Metrics

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A Brief Analysis of the Necessity for Flexible Adjustment of Clenbuterol Detection Proportions in the Pork Slaughter Process HAN Wanyan Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 79-82.   DOI: 10.19978/j.cnki.xmsy.2025.05.12 Abstract （131）      PDF （1031KB）（180）       Knowledge map    Save The Food Safety Law of 2009 and the revised Agricultural Product Quality Safety Law of 2023 both explicitly prohibit the use of lean meat enhancing agents（β ⁃ adrenergic receptor agonists such as clenbuterol， ractopamine，and salbutamol） and mandate compulsory testing during the slaughtering process. The Ministry of Agriculture and Rural Affairs also requires hog slaughtering enterprises to implement the regulation of“pre⁃slaughter testing，”with the testing ratio typically set at 5% to 10%（subject to regional variations）. Under the strict supervision and implementation by various departments，the pass rate of recent sampling inspections has consistently remained above 99%. Given that the positive rate of clenbuterol has long been at an extremely low level and testing costs are high，this study focuses on optimizing the testing ratio for clenbuterol in the hog slaughtering process. It will systematically analyze the current testing situation，policy controversies，scientific basis for ratio adjustments，and optimization suggestions，exploring the necessity and feasibility of dynamically adjusting testing intensity and improving regulatory efficiency while maintaining safety standards. Reference | Related Articles | Metrics

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Comparative Study at Egg Quality of Partridge in Different Ages SHI Liying, GU Jinyuan, LIN Jiezhen, GAN Huadi, WEN Zhenfei, LUO Xingliang, DU Bingwang, HUANG Xunhe Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 33-37.   DOI: 10.19978/j.cnki.xmsy.2025.05.05 Abstract （130）      PDF （1845KB）（351）       Knowledge map    Save This study aimed to investigate the quality differences of eggs from caged partridges at different ages （300 d，500 d，and 800 d）to provide a scientific basis for partridge egg selection and breeding management. By measuring parameters such as egg weight，eggshell weight，egg shape index，eggshell strength，eggshell thickness， Haugh unit， yolk weight， yolk ratio， and albumen height， and performing correlation analysis， the study systematically assessed the changes in egg quality with age. The results showed that：（1）Egg weight and eggshell thickness significantly increased with age（ P<0.05），whereas eggshell weight，eggshell strength，and yolk ratio first increased and then decreased，peaking at 500 d；（2）The Haugh Unit of eggs at 300 d was the highest（83.68 HU）；（3） The egg shape index was significantly negatively correlated with eggshell strength（ P<0.05），and the eggshell thickness of eggs at 800 d（0.31 mm）was significantly greater than that of other ages. The study concluded that the eggs from partridges at 500 d had the best comprehensive quality，and it is recommended that in breeding production，high⁃ quality eggs be selected based on egg quality indicators. Reference | Related Articles | Metrics

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A Case Analysis of Differential Diagnosis and Treatment of Bulimia Syndrome in the Red Panda ZHANG Malong, SA Jiaqi, HUANG Kang, XIE Weijun, ZENG Fanwen Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 91-94.   DOI: 10.19978/j.cnki.xmsy.2025.05.14 Abstract （127）      PDF （1032KB）（127）       Knowledge map    Save Through clinical symptom observation，blood routine and biochemical tests，and fecal parasitological examination，a case of suspected bulimia in a red panda（Ailurus fulgens） was investigated and retrospectively analyzed using dialectical treatment. In mid��May 2024，a red panda exhibited clinical symptoms such as irritability， marked anorexia，and progressive emaciation. The blood routine and biochemical tests showed that the blood glucose （GLU）and urea（UREA）were significantly higher than the normal reference values. Based on the comprehensive analysis of various tests，it was concluded that the patient had bulimia nervosa. Traditional Chinese medicine treatment was adopted，and the syndrome was diagnosed as excessive heart fire and stomach fire，insufficient kidney yin，and disharmony between heart and kidney. The prescription selected was modified Huanglian Ejiao Jizi Decoction，which cleared the heart fire and nourished the kidney yin. After about 20 days of treatment，the red panda showed normal feeding and activity，and its body condition gradually recovered. Reference | Related Articles | Metrics

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Analysis of the Current Situation and Prospects of the Yak Industry Development in Nyingchi City LUOSANG DanDa, OU Zhu, CAO Changren Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 111-115.   DOI: 10.19978/j.cnki.xmsy.2025.04.17 Abstract （125）      PDF （997KB）（26）       Knowledge map    Save This paper conducts a comprehensive analysis of the current state of the yak industry in Nyingchi City，delving into the challenges it confronts and proposing strategic measures for its advancement. The article highlights the pivotal role of the yak industry within the economic，cultural，and social fabric of Nyingchi City，tracing the evolution of the sector. It focuses on analyzing the scale and distribution of yak farming，as well as the structure of the industrial chain. In response to issues such as natural environmental constraints，lagging farming techniques，and slow market and brand development that the yak industry encounters during its growth，the paper proposes development recommendations including enhancing farming technology，strengthening industrial chain integration， promoting breed improvement，and reinforcing brand building. Additionally，the article offers a perspective on the future development prospects of the yak industry in Nyingchi City. Reference | Related Articles | Metrics

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Development of Indirect ELISA Method for Antibody Detection of Classical Swine Fever Virus YAN Shiqi, LI Fukun, QI Dongmei, LAI Yuehui, HUANG Fuqiang, FENG Yuxin, ZHOU Xiaomin, FAN Quanbao Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 66-72.   DOI: 10.19978/j.cnki.xmsy.2025.05.10 Abstract （123）      PDF （2373KB）（58）       Knowledge map    Save Classical swine fever（CSF）caused by classical swine fever virus（CSFV），is a highly contagious and lethal viral disease in swine，for which vaccination remains a critical preventive strategy，antibody levels is an important indicator for evaluating the immune effect of immunized pig herds. In this study，the recombinant E2Y protein of CSFV was successfully expressed using a prokaryotic expression system. The purified E2Y protein served as a coating antigen，and an indirect ELISA method for detecting CSFV⁃specific antibodies was established through the systematic optimization of experimental conditions. Results indicated that the optimal coating concentration of the E2Y protein was 0.25 μg/mL. The assay demonstrated no cross⁃reactivity with positive sera against common swine viruses and exhibited high specificity，sensitivity，and reproducibility. The concordance between this method and a commercial ELISA kit reached 91.67% . This study successfully developed an indirect ELISA for CSFV antibody detection，providing a technical basis for further optimization and development of CSFV antibody detection reagents. Reference | Related Articles | Metrics

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Research Progress on Prevention Treatment of Porcine Viral Infectious Diseases with Traditional Chinese Medicine#br# MA Chunxia, ZHANG Yang, LIU Xueshuang, LI Cuiyun, WU Ya, PAN Qin, REN Shaoke, OU Yunwen Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 6-13.   DOI: 10.19978/j.cnki.xmsy.2025.06.02 Abstract （121）      PDF （1119KB）（48）       Knowledge map    Save Abstract：In recent years，with the rapid development of China 's pig industry. With the expansion of breeding and transportation scale，porcine reproductive and respiratory syndrome virus （PRRSV），porcine circovirus 2 （PCV2），porcine pseudorabies virus（PRV）and porcine epidemic diarrhea virus（PEDV）are becoming more and more prevalent. It has caused huge economic losses to the pig industry. As a traditional Chinese medicine，traditional Chinese medicine has a long history of use. It has the effects of antiviral，anti⁃inflammatory and enhancing immunity. These effects together constitute the cornerstone of the core strategy of traditional Chinese medicine for the treatment of viral infections. With the deepening of related research，traditional Chinese medicine has achieved remarkable results in the prevention and treatment of PRRSV，PCV2，PRV and PEDV，and has broad application prospects. Therefore，this paper reviews the application of traditional Chinese medicine in the prevention and treatment of porcine viral infectious diseases，in order to provide reference for the prevention and treatment of porcine viral infectious diseases and the development of new antiviral Chinese veterinary drugs. Reference | Related Articles | Metrics

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Effect of Valine to Lysine Ratio in Low ⁃ protein Diet on Cytokine and Microorganisms in the Milk of Multiparous Sows WEI Siyong, GU Fang YE Xiangyang, Yang Xuefen, WANG Li, XIAO Hao, Dong Chunlin Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 17-23.   DOI: 10.19978/j.cnki.xmsy.2025.05.03 Abstract （120）      PDF （3986KB）（307）       Knowledge map    Save To reduce feed costs，improve the breeding efficiency of sows，and promote sustainable development in the pig industry，this study investigated the effects of varying valine to lysine（Val/Lys） ratios in low⁃protein diets on the immune function of sows and the microbiota in their milk. Sixty sows with similar body conditions and mating times（fourth parity Landrace × Large White）were selected as the experimental subjects. The minimum nutritional requirements for lactating sows per NRC standards were used as the standard for low⁃protein diets. The sows were randomly divided into six groups：a normal protein group（17% CP，Val/Lys = 0.87）and five low⁃protein diet groups （14.3% CP，Val/Lys ratios of 0.64，0.74，0.84，0.94，and 1.04）. Each sow was accompanied by 12 piglets，and the experiment lasted from the first day of lactation to the 21st day of weaning，a total of 21 days. The results showed that low⁃protein diets without additional valine decreased（ P<0.05）the abundance of the B⁃cell marker CD11b in the mammary glands of sows，while increasing the Val/Lys ratio in low⁃protein diets increased（ P<0.05）the abundance of CD11b in the mammary glands. Low⁃protein diets without additional valine decreased（ P<0.05）the concentration of SIgA in colostrum，while increasing the Val/Lys ratio in low⁃protein diets increased（ P<0.05）the concentration of SIgA in colostrum. Low ⁃ protein diets without additional valine significantly decreased （ P<0.05） the relative abundance of Bacteroidetes， Fusobacteria， and Acidobacteria at the phylum level and Fusobacterium and Staphylococcus at the genus level in the milk microbiota of lactating sows，while increasing the Val/Lys ratio in low⁃ protein diets increased the relative abundance of these microbiotas. In conclusion，low⁃protein diets without additional valine impair the cytokine and microbial diversity of sows，and increasing the Val/Lys ratio in low⁃protein diets can mitigate these negative effects and help maintain the production performance of sows. Reference | Related Articles | Metrics

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Epidemic Status, Transmission Mechanism and Prevention and Control Strategies of Chikungunya Fever QIAN Yanhong, ZHANG Pian, CHEN Jing, ZHANG Xiaoxiao, WANG Xiaohu Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 20-28.   DOI: 10.19978/j.cnki.xmsy.2025.06.04 Abstract （119）      PDF （1272KB）（36）       Knowledge map    Save Chikungunya fever is an acute mosquito⁃borne infectious disease caused by the Chikungunya virus（CHIKV） and transmitted primarily by Aedes mosquitoes. The main clinical manifestations include fever，rash，and joint and muscle pain. The disease exhibits distinct epidemiological features and urban transmission cycles，posing a high infection risk to human populations. Factors such as viral genetic mutations，accelerated globalization，and climate warming have driven the global spread of chikungunya fever in recent years. To date，chikungunya outbreaks have been reported in 119 countries and regions，with an estimated annual cumulative case count exceeding 500 000. Due to the absence of approved commercial vaccines or effective antiviral therapies，Chikungunya fever continues to pose a significant threat to human health，public health systems，and socioeconomic development. Currently，the global spread of chikungunya fever is becoming increasingly severe. Notably，the 2025 outbreak in Foshan，Guangdong��linked to imported cases and subsequent local transmission�� has underscored the urgent need for enhanced prevention and control measures in China. This paper systematically reviews the global and domestic epidemiological status of chikungunya fever，its transmission routes and local transmission characteristics，underlying mechanisms and influencing factors，as well as comprehensive prevention and control strategies. The study aims to provide valuable insights for the scientific prevention and control of epidemic importation and spread in China and for safeguarding public health. Reference | Related Articles | Metrics

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Effects of three plant extracts on growth performance，body composition and serum biochemistry of Litopenaeus vannamei LIU Xiaochun, HU Junru, ZHOU Donglai, HUANG Wen, WU Han, FU Bing, XING Dongxu, CHEN Bing Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 38-44.   DOI: 10.19978/j.cnki.xmsy.2025.05.06 Abstract （115）      PDF （1114KB）（110）       Knowledge map    Save The purpose of this experiment was to study the effects of three plant extracts，soybean isoflavones， mulberry leaf flavonoids and mulberry leaf crude extract，on the growth performance，body composition and serum biochemical indexes of Litopenaeus vannamei. The basal diet（G0，control group）was supplemented with 400 mg/kg soybean isoflavone（G1），50 mg/kg mulberry leaf flavonoid（G2），and 300 mg/kg mulberry leaf crude extract（G3）to formulate experimental diets. A total of 480 Litopenaeus vannamei with an initial body weight of（1.80±0.02）g were randomly allocated into 4 groups with 3 replicates per group and 40 shrimps per replicate. The breeding test period was 60 days. The results showed that：1）There were no significant differences in final body weight，weight gain rate， survival rate，specific growth rate and food intake of Litopenaeus vannamei among all groups（ P>0.05）；the feed coefficient of Litopenaeus vannamei in G1，G2 and G3 groups was lower than that in G0 group，and that in G3 group was significantly lower than that in G0 group（ P<0.05）；2）There was no significant difference in crude protein，crude fat and water content of whole shrimp in each group（P>0.05），but the crude ash content of G2 group was significantly higher than that of G3 group（ P<0.05）；3）There was no significant difference in serum albumin，albumin/globulin， globulin， total protein， triglyceride， alanine aminotransferase， blood glucose， aspartate aminotransferase， cholesterol，high density lipoprotein cholesterol and low density lipoprotein cholesterol levels among the groups（ P> 0.05）；the serum urea content in G2 group was significantly lower than that of G0 group（ P<0.05）. In summary，the addition of 400 mg/kg soybean isoflavone，50 mg/kg mulberry leaf flavonoids and 300 mg/kg mulberry leaf crude extract to the feed could reduce feed coefficient，improve feed utilization rate，and lower serum urea content. Comprehensive evaluation，the application effect of mulberry leaf crude extract is the best. It provides a scientific basis for the application of plant extracts in Litopenaeus vannamei feed. Reference | Related Articles | Metrics

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Progress in the Prevention and Control of Mycoplasma hyopneumoniae ZHOU Hongchao, JIA Pei Guangdong Journal of Animal and Veterinary Science    2026, 51 (1): 1-5.   DOI: 10.19978/j.cnki.xmsy.2026.01.01 Abstract （114）      PDF （1503KB）（63）       Knowledge map    Save Mycoplasmal pneumonia of swine （MPS） is a chronic，wasting infectious disease caused by Mycoplasma hyopneumoniae（Mhp），characterized mainly by coughing and dyspnea. It is also known as swine enzootic pneumonia（EP）or swine chronic respiratory disease. With the intensification of large?scale farming，the infection rate of this disease in domestic pig farms remains high. Although Mhp infection has a low mortality rate，it can increase the culling rate of infected pigs，raise the feed?to?meat ratio，reduce daily weight gain，slow growth，and extend the time to market for finishing pigs，severely undermining the economic efficiency of swine herds. Conventional preventive and control measures for Mhp include vaccination and antibiotic treatment. However，the immune evasion mechanisms formed by Mhp after invading the host，secondary infections，and antibiotic resistance significantly diminish the effectiveness of both Vaccination and antibiotic therapy，making it difficult to eradicate Mhp once it enters a pig herd.To address this challenge，this paper discusses the current status of Mhp infection and control from four aspects：the harmful effects of Mhp，its pathogenic mechanisms，epidemiology，and preventive measures，with the aim of providing a reference for the control of this disease. Reference | Related Articles | Metrics

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Research and Application Effects of Egg��type Quail Feeding Farming Technology DONG Liting, LI Xiang, LI Xingmu, WANG Zheng Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 73-78.   DOI: 10.19978/j.cnki.xmsy.2025.05.11 Abstract （110）      PDF （1042KB）（116）       Knowledge map    Save Abstract：This paper explores the latest advancements in in quail egg production technology and their practical applications. The study focuses on several key areas，including precision nutrition management，bioactive calcium development，improved chick �� rearing systems（such as smart environment control and disease early �� warning systems），enhanced stress �� mitigation strategies，optimized evaporative cooling，and solutions to reduce feather pecking behavior. These integrated technologies have significantly improved farming efficiency and egg quality，while addressing critical challenges in traditional quail farming，such as low feed conversion rates and inadequate disease control. The application of these technologies has enhanced husbandry and egg quality，demonstrating notable progress in overcoming the limitations of conventional practices，particularly in feed utilization and disease management. Reference | Related Articles | Metrics

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Diagnostic Case Report of Partial Rectal Stenosis in the Cat Lin Ran, Kang HuaHua, Wu JunYi, Han Yixin, Fu YunFei, Xu ZhiHong Guangdong Journal of Animal and Veterinary Science    2025, 50 (5): 95-101.   DOI: 10.19978/j.cnki.xmsy.2025.05.15 Abstract （108）      PDF （2890KB）（133）       Knowledge map    Save There are numerous factors contributing to partial rectal stenosis in cats，including congenital and acquired ones. Common clinical symptoms include abnormal defecation，abdominal distension and pain，and intestinal obstruction. Partial rectal stenosis can be preliminarily diagnosed through computed tomography（CT）and magnetic resonance imaging（MRI），but a definitive diagnosis requires histopathological examination. This paper details the examination process of the affected cats，the surgical plan，and the postoperative recovery situation. Before the surgery，computer tomography（CT），magnetic resonance imaging（MRI），ultrasound，cat serum amyloid A （fSAA），biochemical and blood tests were used to assist in diagnosis. During the surgery，the diseased tissue was removed and sent for histopathological examination. The postoperative recovery was tracked and the diagnosis process was analyzed to provide clinical reference. Reference | Related Articles | Metrics

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Observation on the Anesthetic Effect of Dexmetomidine Combined with Alfaxalone on Trachemys scripta elegans PENG Shiming, XIE Weijun, HUANG Kang, ZENG Yuhui, CHENTAN Zipeng Guangdong Journal of Animal and Veterinary Science    2025, 50 (4): 78-82.   DOI: 10.19978/j.cnki.xmsy.2025.04.13 Abstract （105）      PDF （1703KB）（146）       Knowledge map    Save In order to explore the clinical anesthesia drug combination and anesthesia effect of aquatic turtles，a trial was conducted using Dexmedetomidine（0.05 mg/kg）combined with Afaxalone（5 mg / kg）to anesthetize nine Trachemys scripta elegans.The time and behavioral manifestations of the experimental animals during the induction period，anesthesia period and recovery period were recorded respectively to evaluate the effects of the drugs on sedation，analgesia and muscle .Blood tests and detection of body indicators such as heart rate were conducted on the experimental animals to assess the effect of anesthesia on animal physiological constants. The results showed that the combination of the drugs has an anesthesia induction period of（12.11±2.86）min for Trachemys scripta elegans，and can provide an effective anesthesia induction of（51.89±7.21）min. During the anesthesia process，the experimental animals was stable and showed no adverse reactions. After intramuscular injection of the Atipamezole（0.5 mg/kg），the animals fully recovered after（18.22±4.42）min. The overall effect of anesthesia operation on the blood biochemical indicators and heart rate of the experimental was not significant，indicating that this combination and dosage are suitable for clinical anesthesia of aquatic turtles. Reference | Related Articles | Metrics

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A Case Report of Angiostrongylus cantonensis Infection in a Dog CHEN Huayuan, CHEN Siyuan, LI Qing, GONG Mofeng, YU Jiezhou, LI Shaochuan Guangdong Journal of Animal and Veterinary Science    2025, 50 (6): 112-115.   DOI: 10.19978/j.cnki.xmsy.2025.06.18 Abstract （104）      PDF （2949KB）（131）       Knowledge map    Save Canine neurla angiostrongylosis （CNA） is a parasitic disease caused by the infection with Angiostrongyliasis cantonensis，characterized primarily by severe neurological dysfunction. This article discusses the diagnosis，prevention and control of CNA，aiming to provide a reference for clinical practice. A 5⁃year⁃old male Bichon Frise was presented with paralysis after reportedly ingesting a giant African land snail. Diagnostic investigations included clinical examination，complete blood count，cerebrospinal fluid（CSF）analysis，magnetic resonance imaging（MRI），and polymerase chain reaction（PCR）testing. CSF smear examination revealed elongated structures morphologically consistent with parasitic nematodes. MRI findings included T2 ⁃ weighted hyperintense signals in the thoracolumbar spinal cord and mild leptomeningeal enhancement in the brain. Based on these findings， the history of possible snail ingestion，and the endemic geographical location，a definitive diagnosis of A. cantonensis infection was confirmed. The dog died three days after presentation without receiving treatment. This case indicates that canine neural angiostrongylosis is rapidly progressive and carries a high mortality risk，underscoring the need for prompt clinical recognition and effective diagnostic and therapeutic interventions. Reference | Related Articles | Metrics