Abstract: To explore the detection effect of different detection methods and the feasibility of the combination of avian leukemia virus（ALV）infection and AL purification，the study in chicken，using ALV p27 antigen ELISA method and ALV⁃JPCR nucleic acid detection method，from 2020 to 2024 a total of 1 333 randomly collected whole blood samples for ALV detection analysis. The results showed that in Tibetan chickens，the positive rate of ALV p27 antigen ELISA test was 15.42%，ALV⁃JPCR nucleic acid test was 2.49%. The total positive rate of the two detection methods was 17.23%，among which only 3 individuals were positive for the two detection methods，accounting for 3.95% of the total positive individuals. In addition，since AL purification in 2020 to 2024，the AL positive rate of milin Tibetan chickens decreased from 17.23% to 0 between 2020 and 2024. The results show that there are mixed infection of ALV⁃J subgroup and other subgroups in the flock；the combination of the two methods can greatly improve the ALV detection rate and can provide reference for the actual purification work；the AL purification of milin Tibetan chicken group is significant，which basically realizes the effective control of AL in the flock，which can provide data support for the AL prevention and control purification of this species.

Key words: Milin Tibetan chicken, Avian leukosis, PCR nucleic acid test, p27 antigen ELISA test

摘要： 为探究禽白血病（AL）不同的检测方法的检测效果及其组合使用的可行性以及原种米 林藏鸡群体的禽白血病病毒（ALV）感染和AL净化情况，该研究以米林藏鸡为对象,采用ALV p27 抗原ELISA检测法和ALV?J PCR核酸检测法，对2020至2024年共计1 333份随机采集的全血样 品进行ALV检测分析。结果显示，曾祖代米林藏鸡中，ALV p27抗原ELISA检测法检出阳性率为 15.42%，ALV?J PCR核酸检测法检出阳性率为2.49%，两种检测方法检出总阳性率为17.23%，其 中两种检测方法检测结果均呈阳性反应的个体仅有3只，占总阳性个体的3.95%。此外，自2020 年开展AL净化至2024年，米林藏鸡群体AL阳性率在2020?2024年间从17.23%降至0。结果表明， 曾祖代米林藏鸡群体中存在ALV?J亚群与其他亚群的混合感染；两种方法组合使用检测能够大大 提高ALV检出率，可为实际净化工作提供参考；米林藏鸡群体AL净化的进展较为显著，基本实 现了鸡群中AL的有效控制，可为该鸡种的AL防控净化提供数据支持。

关键词: 米林藏鸡, 禽白血病, PCR核酸检测, p27抗原ELISA检测