|
|
Effects of Different Temperatures on Viability and Sporulation of Oocysts from Eimeria Tenella
LIN Xuhui#, ZHU Yibin#, GUO Suyin, QI Nanshan, GUO Zhaowen, LI Juan , YAN Zhuanqiang , YINLijuan, LI Ruhong, LIU Zuozheng, LV Minna, LIAO Shenquan, SUN Mingfei
Guangdong Journal of Animal and Veterinary Science
2023, 48 (4):
13-19.
DOI: 10.19978/j.cnki.xmsy.2023.04.03
Temperature is an important factor affecting the sporulation and viability of avian coccidia oocysts. In this study,the effects of different temperatures on the sporulation of Eimeria tenella unsporulated oocysts and the viability of sporulated oocysts were investigated by monitoring the sporulation rate of avian coccidia oocysts,intestinal lesions and oocyst production. The results showed that there was no significant change in the oocyst sporulation activity compared to be treated at -80 ℃ for 30 min,and at -40 ℃,-20 ℃,0 ℃,4 ℃,10 ℃,28 ℃,40 ℃,50 ℃ for 30 min or 1 h respectively,but the oocyst sporulation activity was inhibited after administrated at -80 ℃ for more than 1 h,- 40 ℃ or - 20 ℃ for more than 48 h,and 60 ℃ for more than 30 min. The sporulated oocysts were killed by exposure to liquid nitrogen for 2 min,or at -80 ℃ or higher than 50 ℃ for more than 1 h,whereas sporulated oocysts remain viable at -20 ℃ for 1 to 12 h or at 50 ℃ for 30 min. This study provides data support for the storage conditions of avian coccidia oocysts,and has certain research significance for the prevention and treatment of avian coccidiosis.
Reference |
Related Articles |
Metrics
|
|