Abstract: To investigate tick species diversity，major tick?borne pathogens，and their seasonal dynamics in a dog breeding facility in Guangdong，this study randomly collected tick samples from various adult dogs and the surrounding environment. Molecular biological detection （PCR and nested PCR） was employed，followed by bioinformatic analysis of genetic markers such as 16S rRNA or 18S rRNA to systematically characterize pathogen types and seasonal distribution patterns. Results showed that the predominant tick species was Rhipicephalus sanguineus （belonging to the family Ixodidae，genus Rhipicephalus），mainly active from May to September，peaking in July， with markedly reduced activity in winter. Mixed infections with multiple pathogens were detected，primarily involving Babesia，Ehrlichia，and Anaplasma. Based on these findings，an integrated strategy for the prevention and control of tick?borne diseases was proposed，centered on“environmental intervention—chemical control—health monitoring”， providing a scientific reference for the management of similar dog breeding facilities.

Key words: Dogs；Ticks；Tick?borne pathogens；Integrated control

摘要： 为明确广东某犬场蜱虫种类、主要蜱媒病原及季节流行特征。研究通过随机采集不 同类型成年犬及周边环境中的蜱虫样品，采用PCR及巢式PCR技术进行分子生物学检测，并 基于16S rRNA与18S rRNA等基因片段进行序列比对与生物信息学分析，系统解析了该犬场蜱 虫携带病原的种类及其季节性分布规律。结果显示，该犬场蜱以硬蜱科扇头蜱属的血红扇头 蜱（Rhipicephalus sanguineus）为主，主要活动期为5月-9月，7月为发生高峰，冬季活动显著减 少。该犬场存在多种病原混合感染现象，其中以巴贝斯虫（Babesia）、埃立克体（Ehrlichia）和无 形体（Anaplasma）为主要流行病原。基于上述结果，研究提出以“环境干预?化学防治?健康监 测”为核心的综合性蜱媒传染病防控策略，为该类犬场的科学防控提供依据。

关键词: 犬；蜱虫；蜱媒病原；综合防控