关键词: 壳聚糖?蒙脱土插层复合物；呕吐毒素；复合脱毒剂；生长性能；肠道微生物

Abstract: This experiment was based on the chitosan ? montmorillonite intercalation composite （Chitosan ? montmorillonite，CS ? MMT），and incorporated probiotics，enzymes and yeast cell walls to develop a CS ? MMT composite detoxification agent（CDA）with the functions of adsorption and degradation of deoxynivalenole. Thirty?six healthy male C57BL/6J mice at 5 weeks of age were selected and randomly divided into 6 groups（n=6）：normal control group，DON treatment group（2 mg/kg），CDA alone treatment group（100 mg/kg），CDA low?dose group （50 mg/kg + DON），CDA medium?dose group（100 mg/kg + DON），and CDA high?dose group（200 mg/kg + DON）. The mice were given continuous treatment for 21 days and their body weight，daily feed intake，daily water consumption，and DON residue in feces were recorded. After the experiment，blood serum and intestinal contents of the mice were collected. It was found that CDA treatment significantly improved various growth performance indicators of DON?exposed mice and the residual amount of DON in their feces，and reduced the levels of pro?inflammatory factors in their serum. Moreover， CDA increased the richness of the intestinal microbiota through multiple mechanisms，promoted the growth of beneficial bacteria and inhibited the reproduction of harmful bacteria. CDA can improve the intestinal health of DON?exposed animals，providing a new approach to alleviating the harm caused by DON contamination to animal health.

Key words: Chitosan?montmorillonite intercalation complex；Deoxynivalenole；Composite detoxification agent；Growth performance；Intestinal microorganisms