To investigate tick species diversity，major tick?borne pathogens，and their seasonal dynamics in a dog breeding facility in Guangdong，this study randomly collected tick samples from various adult dogs and the surrounding environment. Molecular biological detection （PCR and nested PCR） was employed，followed by bioinformatic analysis of genetic markers such as 16S rRNA or 18S rRNA to systematically characterize pathogen types and seasonal distribution patterns. Results showed that the predominant tick species was Rhipicephalus sanguineus （belonging to the family Ixodidae，genus Rhipicephalus），mainly active from May to September，peaking in July， with markedly reduced activity in winter. Mixed infections with multiple pathogens were detected，primarily involving Babesia，Ehrlichia，and Anaplasma. Based on these findings，an integrated strategy for the prevention and control of tick?borne diseases was proposed，centered on“environmental intervention—chemical control—health monitoring”， providing a scientific reference for the management of similar dog breeding facilities.