Abstract: This study was conducted to study the effects of dietary alfatoxin B1（AFB1）on body composition，liver histomorphology and toxin accumulation of Lateolabrax maculatus. A total of 480 L. maculatus with an initial body weight of（2.90±0.02）g were randomly divided into 4 groups with 3 replicates per group and 40 fish per replicate. Five isonitrogenous and isolipidic diets，that are the basal diet G0（without AFB1），G0.1（adding 0.1 mg/kg of AFB1）， G0.5（adding 0.5 mg/kg of AFB1）and G1.0（adding 1.0 mg/kg of AFB1），were fed to apparent satiation during the 56⁃ day feeding trial. Inclusion of 0.1-1.0 mg/kg AFB1 in diets did not affect body composition of fish（P>0.05），but induced liver tissue injury. With dietary AFB1 increasing，the degeneration and necrosis degree of hepatocytes and fat vacuolization became more serious. AFB1 accumulation in the liver of fish was higher than that in muscle，which was increased with dietary AFB1 increasing（P<0.05）. In conclusion，dietary inclusion of 0.1-1.0 mg/kg AFB1 induced liver tissue injury of L. maculatus. AFB1 accumulation in liver of fish was higher than that in muscle，and was positively correlated with the supplemental level of AFB1 in diets.

Key words: Alfatoxin B1, Lateolabrax maculatus, Histology, Toxin accumulation

摘要： 该文旨在研究饲料中添加黄曲霉毒素B1（AFB1）对海鲈体成分、肝脏组织结构及毒 素累积量的影响。选择初体重为（2.90±0.02）g 的海鲈480尾，随机分为4组，每组3个重复，每 个重复40 尾鱼。在基础饲料G0（不添加AFB1）上分别添加0.1（G0.1）、0.5（G0.5）和1.0 （G1.0）mg/kg的AFB1，配制4组试验饲料。采取饱食投喂，试验周期为56 d。结果表明，各组 间全鱼的水分、粗蛋白、粗脂肪和灰分含量无显著差异（P>0.05）。AFB1可诱导海鲈肝脏组织损 伤，且随着饲料中AFB1含量增加，肝细胞出现变性、坏死及脂肪空泡化的程度越严重。海鲈 肝脏中AFB1的累积量高于肌肉，随着饲料中AFB1添加量的增加，肝脏中AFB1累积量显著升 高（P<0.05）。综上所述，饲料中添加0.1-1.0 mg/kg AFB1可诱导肝脏组织损伤。AFB1在肝脏 中的累积量高于肌肉，且与AFB1添加量呈正相关。

关键词: 黄曲霉毒素B1, 海鲈, 组织结构, 毒素累积量