Guangdong Journal of Animal and Veterinary Science ›› 2025, Vol. 50 ›› Issue (2): 1-7.DOI: 10.19978/j.cnki.xmsy.2025.02.01

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Optimization of the Extraction Process and Analysis of the Antioxidant Activity of Phlorizin from Litchi Shell

MENG Hongru12#XU Shuangyuan12#LIN Zhihao3SUN Mingfei2LIU Wenjun1*QI Nanshan2LI Juan2 ZHANG Xiaoai4LIN Xuhui2LV Minna2CAI Haiming2SONG Yongle2CHEN Xiangjie2ZHU Yibin2 ZHANG Jianfei2LI Yingxin5*LIAO Shenquan2*   

  1. 1.College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering, Guangzhou Guangdong 510225;2. Institute of Animal Health,Guangdong Academy of Agricultural Sciences/Key Laboratory of Avian Influenza and
    Other Major Poultry Diseases Prevention and Control/Key Laboratory of Livestock Disease Prevention of Guangdong Province,Guangzhou Guangdong 510640;3. Guangzhou University of Chinese Medicine,Guangzhou Guangdong 510006;4. Agro⁃biological Gene Research Center of Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640;5. Shenzhen Animal and Plant Disease Prevention and Control Center,Shenzhen Guangdong 518052
  • Online:2025-04-24 Published:2025-04-18

荔枝壳中根皮苷的提取工艺优化及其抗氧化活性分析

孟弘茹1,2#,徐双园1,2#,林之灏3,孙铭飞2,刘文俊1*,戚南山2,李娟2,张晓爱4,林栩慧2,吕敏娜2,蔡海明2,宋勇乐2,陈祥杰2,朱易斌2,张健騑2,李颖鑫5*,廖申权2*   

  1. (1. 仲恺农业工程学院动物科技学院,广东广州510225; 2. 广东省农业科学院动物卫生研究所/农业农村部禽流感等家禽重大疫病防控重点实验室/广 东省畜禽疫病防治研究重点实验室,广东广州510640; 3. 广州中医药大学,广东广州510006; 4. 广东省农业科学院农业生物基因研究中心,广东广州510640; 5. 深圳市动植物疫病预防控制中心,广东深圳518055)
  • 通讯作者: 李颖鑫,廖申权
  • 基金资助:
    广东省农业科学院协同创新中心项目(XTXM202202);科技创新战略专项资金(高水平农科院建设)(202122TD),广州市科技计划项目(2023B04J0137,2023A04J0789)

Abstract: To optimize the extraction process of phlorizin from litchi pericarp and assess its antioxidant activity,we enhanced the extraction efficiency by optimizing several parameters,including the solid⁃liquid ratio,solvent concentration, extraction temperature,solvent pH,and extraction duration. The antioxidant activity of phlorizin was analyzed using the DPPH and ABTS radical scavenging assays. Single⁃factor experimental results indicated the following extraction rates:a solid⁃liquid ratio of 1∶40 yielded 1.22% phlorizin,a 65% ethanol solution resulted in 1.20%,a water bath temperature of 60 ℃ produced 1.51%,and a solvent pH of 5 achieved 1.58%. After an extraction time of 60 min,the yield of phlorizin reached 1.39%. The optimized extraction conditions included a solid􀆼liquid ratio of 1∶40,65% ethanol concentration,60 ℃ temperature,pH 5,and a 60􀆼minute extraction time. These conditions yielded the extraction rate of phlorizin from litchi shell ranged between 1.16% and 1.39%. The antioxidant activity of the extracts demonstrated that the antioxidant capacity (TEAC value)of the phlorizin extract was 0.34 μg TE/μg,with a total antioxidant capacity of(4.02±1.08)mmol/g. These findings provide a scientific basis for developing antioxidant preparations derived from litchi shell.

Key words: Litchi shell, Phlorizin, Extraction process, Antioxidant activity

摘要: 为优化荔枝壳中根皮苷的提取工艺并分析根皮苷提取物的抗氧化活性,该研究通过 优化料液比、溶剂浓度、提取温度、溶剂pH值和提取时间等单因素条件,以提高从荔枝壳中提取 根皮苷的效率,并采用DPPH法和ABTS法分析所提取根皮苷的抗氧化活性。单因素分析试验 结果表明,以1∶40料液比提取根皮苷得率为1.22%,以65%乙醇溶液提取根皮苷得率为1.20%, 在提取温度为60 ℃条件下提取根皮苷得率为1.51%,以溶剂(pH 5)提取根皮苷得率为1.58%, 当提取时间为60 min时根皮苷的得率为1.39%。优化所得提取工艺条件为:料液比1∶40,乙醇 浓度65%,提取温度60 ℃,溶剂pH值5,提取时间60 min,在此条件下荔枝壳根皮苷提取率为 1.16%—1.39%。检测提取物的抗氧化活性发现,根皮苷提取物的抗氧化能力(TEAC值)为0.34 μg TE/μg,总抗氧化能力为(4.02±1.08)mmol/g,表明从荔枝壳中提取的根皮苷具有较好的抗氧 化活性,为开发荔枝壳来源的抗氧化制剂提供理论依据。

关键词: 荔枝壳, 根皮苷, 提取工艺, 抗氧化活性

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