Abstract: To quickly find out the variety characteristics of the new resources of Luokeng chicken，in this study， molecular assisted marker technology involving recessive white⁃feathered （TYR）allele and Z⁃linked dw allele were performed. Consequently，46 heterozygous recessive white⁃feathered individuals were detected，the frequency of the heterozygous recessive white⁃feathered allele in the F0 and F1 generation were 11% and 13.67%，respectively and no Z⁃linked dw allele individual were detected across 356 samples. Altogether，the potential recessive white⁃feathered heterozygotes were totally eliminated within one generation，thus laying a foundation for the further genetic protection of Luokeng chicken.

Key words: TYR allele, dw allele, Molecular assisted marker, Luokeng chicken

摘要： 为了快速摸清罗坑鸡新资源的品种特性，该文利用分子标记辅助技术对罗坑鸡群体进行隐性白羽基因和矮小基因PCR鉴定。在356只群体样本中，检测出含隐性白羽基因杂合型的个体46只，F0、F1世代含隐性白羽基因杂合子频率分别为11%、13.67%,未检测出含矮小基因的个体。利用分子标记辅助技术可快速淘汰杂合子，有效缩短地方种鸡提纯复壮周期，为罗坑鸡种质资源保护评鉴、开发利用奠定理论基础。

关键词: TYR 基因, dw 基因, 分子辅助标记, 罗坑鸡