Abstract: Novel duck reovirus（NDRV）is capable of inducing a contagious disease in waterfowl，including ducks，Beijing ducks，and geese，which is primarily characterized by irregular hemorrhaging and necrosis of the liver and spleen. In recent years，the incidence of this disease in waterfowl in Guangdong Province has been on the rise. In order to understand the genetic evolution characteristics of the novel duck enteritis virus in Guangdong Province，a strain of NDRV was isolated from affected Muscovy ducks in a duck farm in Foshan，Guangdong. Analysis of the σC gene sequence of this strain revealed that it belongs to the same branch as NDRV，with nucleotide similarity ranging from 95.1% to 99.6%. The genetic distance from typical MDRV and ARV was far，with similarities below 50%. Compared to the SH12 and DH13 strains，this strain showed variations in 15 amino acid residues. To understand the antigenicity of the σC protein of this strain，a pET32a⁃σC plasmid was further constructed，and a method for stable expression of recombinant σC protein in BL21（DE3）was established. Western blot analysis confirmed that this protein can specifically bind to NDRV⁃positive serum and rabbit polyclonal antibodies. This study provides a reference for further understanding the genetic evolution characteristics of NDRV in waterfowl in Guangdong Province and the development of gene⁃engineered vaccines.

Key words: Novel duck enteritis virus, σC protein, Genetic evolution analysis, Polyclonal antibody preparation

摘要： 新型呼肠孤病毒（Novel duck reovirus，NDRV）是可引起番鸭、北京鸭和鹅等多种水 禽，发生肝脏、脾脏不规则出血和坏死的传染性疾病，近年来广东地区水禽中该病的发病率呈 上升趋势。为了解广东地区新型呼肠孤病毒遗传进化特征，该试验从广东佛山某鸭场发病番 鸭中分离得到一株试验毒株，通过对该毒株σC 基因序列分析，结果表明该试验毒株与NDRV 同处于一个分支，并且核苷酸相似性达95.1%-99.6%；而与典型MDRV和ARV遗传距离较远， 且相似性均低于50%。与SH12和DH13毒株相比，该试验毒株出现了15个氨基酸残基的变 异。为了解该试验毒株σC蛋白的抗原性，进一步构建了pET32a?σC质粒，并建立了重组σC蛋 白在BL21（DE3）内稳定表达的方法，通过Western blot鉴定该蛋白能够与NDRV阳性血清和兔 多克隆抗体发生特异性结合。为进一步了解NDRV在广东地区水禽中的遗传进化特征以及基 因工程疫苗的研发提供参考。

关键词: 新型鸭呼肠孤病毒, σC蛋白, 遗传进化分析, 多克隆抗体制备