Abstract: In order to determine the cause of death of rong⁃tailed lemurs in a zoo in Guangdong Province，the methods were conducted through clinical autopsy and collection of disease materials，bacterial isolation and culture， morphological observation，16S rRNA gene amplification and sequencing analysis，virulence gene detection and drug susceptibility test. The results showed that a strain of Klebsiella pneumoniae was isolated in this study. The amplified fragment of 16S rRNA gene of the isolated strain was 1459 bp and the sequence similarity with 12 reference strains of Klebsiella pneumoniae on NCBI was more than 99.86% . Virulence gene test result showed that the bacterium contained uge，wabG，aereobctin，khe，rmpA，ureA and fimH virulence genes. The results of drug susceptibility test showed that the isolates were resistant to amoxicillin，penicillin，erythromycin and lincomycin in different degrees. This study provides a basis for the diagnosis and rational drug against Klebsiella pneumoniae in ring⁃tailed lemurs，as well as a reference for the prevention and control of Klebsiella pneumoniae.

Key words: Ring?tailed lemur, Klebsiella pneumonia, Isolation and identification, Drug susceptibility test

摘要： 为明确广东省某动物园的环尾狐猴死亡原因，无菌采集死亡环尾狐猴肺脏组织，采 用细菌分离培养、形态学观察、16S rRNA基因扩增及测序分析、毒力基因检测及药敏试验等检 测方法。结果显示，该试验成功分离到1株环尾狐猴源肺炎克雷伯氏菌，分离株的16S rRNA 基因扩增片段大小为1 459 bp，与NCBI 上12 株肺炎克雷伯氏菌参考菌株序列相似性均在 99.86%以上。毒力基因检测结果显示该分离株含有uge、wabG、aereobctin、khe、rmpA、ureA、fimH 毒力基因。药敏试验结果显示，分离株对阿莫西林、青霉素、红霉素、林可霉素表现出不同程度 的耐药。该研究结果为环尾狐猴源肺炎克雷伯氏菌的检测、诊断及治疗提供了参考依据，以及 为肺炎克雷伯氏菌防控提供了研究基础。

关键词: 环尾狐猴, 肺炎克雷伯氏菌, 分离鉴定, 药敏试验