High?throughput sequencing（HTS）has been increasingly applied in animal disease diagnosis and molecular epidemiological investigations in recent years. In intensive swine production systems，clinical syndromes such as respiratory disease，diarrhea，and reproductive disorders are often associated with multiple co ? infecting pathogens，making comprehensive pathogen identification difficult using conventional targeted diagnostic assays. By enabling large ? scale parallel sequencing of nucleic acids in clinical samples，HTS allows unbiased detection of pathogens and provides high ? resolution genomic information. This capability offers significant advantages for the discovery of emerging pathogens，characterization of mixed infections，analysis of viral genetic variation，and epidemiological source tracing. In this review，we summarize recent advances in the application of HTS technologies for viral detection in swine diseases. Particular attention is given to three major sequencing strategies—Whole?Genome Sequencing（WGS），Metagenomic Next?Generation Sequencing（mNGS），and Targeted Next?Generation Sequencing （tNGS）—and their applications in the detection of major swine viruses. In addition，the potential of HTS for swine disease surveillance and precision disease control is discussed.

In order to establish a rapid and accurate detection method for African swine fever（ASF），a standarddetection of ASFV，it was found that the primers and probes provided by CADC were more sensitive to the detection of ASFV，and the DNA extraction kit of Axygen brand was more cost?effective for the detection of ASFV. plasmid and a standard curve were constructed for the late translation protein p72 gene of ASFV. The sensitivity of primers and probes provided by OIE and CADC was compared by fluorescence quantitative PCR technology，and the detection efficiency of ASFV DNA extracted by various DNA extraction kits was evaluated，providing data reference for the efficient detection of ASFV. The results showed that p72 standard plasmid had better detection efficiency and sensitivity among the standard plasmid and standard curve of p72 gene of ASFV constructed. Through the use of primers， probes and various DNA extraction kits provided by OIE and CADC for fluorescence quantitative PCR