In recent years，porcine rotavirus（PoRV）has shown a high incidence trend worldwide and has surpassed traditional pathogens such as porcine epidemic diarrhea virus（PEDV）and transmissible gastroenteritis virus（TGEV）to become the dominant cause diarrhea in piglets. The virus exhibits high tissue tropism，primarily infecting the small intestinal mucosal epithelial cells of piglets from birth to weaning（1-6 weeks of age）. Clinically， infected piglets present with markedly increased defecation frequency and pass watery，light yellow or grayish?white feces，accompanied by rapid dehydration and electrolyte imbalance，ultimately leading to death from circulatory failure. Furthermore，intestinal barrier damage caused by the virus predisposes affected piglets to secondary infections by other pathogenic bacteria，with morbidity rates in piglet populations reaching as high as 100%. Currently，the prevalence of porcine rotavirus has become a key factor restricting the sustainable development of the pig farming industry. The segmental nature of the viral genome facilitates recombination with rotaviruses from other host species. In recent years，the identification of human? porcine recombinant strains and novel native variants has raised concerns regarding potential zoonotic transmission. This article systematically reviews the etiological characteristics， epidemiology and genetic variations，pathological changes and pathogenic mechanisms，diagnostic methods，and vaccine development progress associated with porcine rotavirus，with the aim of providing a comprehensive overview of current research advancements and establishing a theoretical basis for assessing the risk of cross?species transmission of this virus.

In order to find an alternative laboratory method for the efficacy test of classical swine fever live vaccine （cell line origin），in this study the qualified vaccine was 10 times gradiently diluted with special diluent by doses and inoculated into the cell plate covered with swine testicles cells and cultured for 72 h. The dilution of the positive serum of classical swine fever virus（CSFV）and the goat anti ? pig IgG（FITC marker）was determined as 1∶400 both. A method of indirect immunofluorescence assay（IFA）was established for detection of classical swine fever live vaccine （cell line origin），this method has good sensitivity，specificity and reproducibility. The IFA method was used to detect 10 batches of classical swine fever live vaccine（cell line origin），the results were 104.67~105.33TCID50/dose. The established IFA detection method can provide technical support to quality control of classical swine fever live vaccine （cell line origin）.

A virus suspected to be porcine pseudorabies virus（PRV）was isolated from dead pigs in Guangdong province. The typical cytopathic effect（CPE）could be observed when the isolated virus was cultured on the swine testicular cells and it’s tissue culture infectious dose（TCID50）was 106.8/0.1 mL. A specific 370 bp long DNA fragment could be expanded using polymerase chain reaction（PCR）analysis from the isolated virus DNA. The sequence analysis results showed that the nucleotide identity of gE ? gene of PRV GD strain were between 97.6% ～99.9% comparing with several domestic and foreign PRV strains published in GenBank. The isolated virus strain was confirmed to be porcine pseudorabies virus and named as PRV GD strain. Inoculation of mice、rabbits、piglets and fattening pigs can cause the disease and death with strong pathogenicity.