Abstract: To determine the growth curve，blood physiological and biochemical parameters and main organ weights of C57BL/6 mice introduced and produced in our center，so as to provide basic reference data for the application of C57BL/6 mice. The body weight of 80 SPF C57BL/6 mice（equal male and female）aged 3－8 weeks was measured，and the growth curve was plotted. Twenty C57BL/6 mice（5 - and 10-week-old，equal male and female）were randomly selected for in vivo weighing. Following anesthesia，blood was collected via retro-orbital puncture for hematological and biochemical analyses，and major organs were dissected and weighed. Body weight increased with the age，with rapid growth at 3－5 weeks before gradually stabilizing. Significant differences（P<0.01 or P<0.05） were observed in certain hematological parameters，biochemical indices，organ weights，and organ coefficients between sexes within the same age group and between age groups within the same sex. The results of this study provided basic reference data for the application of C57BL/6 mice. It was found that week age and gender had certain effects on blood physiological and biochemical indexes，major organ weight and organ coefficient of C57BL/6 mice. Attention should be paid to the selection of week age and sex when using C57BL/6 mice.

Key words: C57BL/6 mice；Growth curve；Blood physiological and biochemical indexes；Organ weight；Organ coefficient

摘要： 该研究测定该中心引进生产的C57BL/6小鼠的生长曲线、血液生理生化指标值及主要脏器重量，为C57BL/6小鼠的应用提供基础参考值。称取80只（雌雄各半）SPF级C57BL/6小鼠3－8周龄体重，绘制生长曲线；随机选取5、10周龄C57BL/6小鼠各20只（雌雄各半），活体称重，麻醉后摘眼球采血后测定血常规、血生化指标值，并剖取主要脏器称重。C57BL/6小鼠的体重随着日龄的增长而增加，3－5周龄增长较为迅速，之后逐渐变缓并趋于稳定；血常规指标、血生化指标、主要脏器重量、脏器指数方面，同性别不同周龄、同周龄不同性别之间比较均有部分指标存在显著性差异（P<0.01或P<0.05）。此研究结果为C57BL/6小鼠的应用提供了基础参考数据，研究发现周龄、性别均对C57BL/6小鼠血液生理生化指标、主要脏器重量、脏器指数有一定影响，在使用C57BL/6小鼠时应注重对周龄和性别的选择。

关键词: C57BL/6小鼠；生长曲线；血液生理生化指标；脏器重量；脏器指数