Abstract: To investigate the genetic variation of prevalent feline calicivirus（FCV）strains circulating in China， ocular and nasal swabs were collected from cats with suspected FCV infection in Henan and Tianjin provinces/ municipalities for virus isolation. The isolated strains were subjected to purity，specificity，viral load and VP1 gene sequencing. The results showed that all 15 FCV isolates exhibited satisfactory purity and specificity，with viral loads no lower than 107.5TCID50/mL. Sequence analysis of the VP1 gene revealed that the nucleotide homology of the VP1 gene among the 15 FCV strains ranged from 72.8% to 99.9%，and the nucleotide homology of those strains with 18 published domestic and foreign FCV reference strains retrieved from GenBank ranges from 73.0% to 87.1%。Based on genetic differences in the sequences，these strains were classified into two genotypes，GⅠ and GⅡ，including 13 GⅠstrains（accounting for 86.7%）and 2 GⅡ strains（accounting for 13.3%）. In addition，all isolated strains displayed substantial genetic divergence from three classic GⅠvaccine reference strains. The above findings provide fundamental data for further elucidating the genetic variation of prevailing FCV strains in China and optimizing vaccine selection strategies.

Key words: Feline；Calicivirus；Isolation and identification；Phylogenetic

摘要： 为了解我国猫杯状病毒流行毒株的遗传变异情况，采集河南、天津两地疑似猫杯状 病毒感染猫的眼鼻拭子进行病毒分离，并对分离毒株进行了纯净性、特异性、病毒含量以及 VP1基因测序分析。结果显示：15株FCV分离毒株纯净性、特异性均良好，每毫升病毒含量均 不低于107.5TCID50。VP1 基因序列分析结果表明：15 株猫杯状病毒分离株VP1 基因核苷酸同源 性为72.8%—99.9%，与Genbank 中已发表的18 株国内外FCV 参考毒株同源性为73.0%— 87.1%，并根据其序列遗传差异分可为GⅠ、GⅡ两个基因型，其中GⅠ型毒株13 株（占比 86.7%），GⅡ型毒株2株（占比13.3%），且全部分离毒株与3株经典GⅠ型疫苗参考株遗传距离 均较远。上述研究结果为深入了解我国猫杯状病毒流行毒株遗传变异情况、优化疫苗选型提 供了数据参考。

关键词: 猫；杯状病毒；分离鉴定；遗传进化